Protocol Optimization for Long-Read 16S Metagenomic Sequencing
Long-read 16S sequencing has become an increasingly useful approach for characterizing bacterial and archaeal communities when researchers need more sequence information than conventional short-variable-region amplicon sequencing can provide. The bacterial and archaeal 16S rRNA gene is approximately 1.5 kb long and contains conserved regions interspersed with variable regions that provide taxonomic information. Long-read workflows can…